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Characterisation of Fermented Grain-based Raw Materials and Foods

Analytical Methods from the HealthFerm Project

Arno G. B. Wouters (Editor), Yamina De Bondt (Editor), Kati Katina (Editor), Christophe M. Courtin (Editor),

Category: Science, Engineering and Technology

Language: English

DOI: 10.11116/9789461667410

ISBN: 9789462705111

Publication date: February 25, 2026

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Number of pages: 272

Size: 234 x 156 x 16 mm

Number of illustrations: 50

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Funded by: KU Leuven Fund for Fair Open Access

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Category: Science, Engineering and Technology

Language: English

DOI: 10.11116/9789461667410

ISBN: 9789461667410

Publication date: February 25, 2026

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Number of pages: 272

Number of illustrations: 50

Illustrations and other content description:
Figures, tables, schemes Guaranteed Peer Reviewed Content

Funded by: KU Leuven Fund for Fair Open Access

Category: Science, Engineering and Technology

Language: English

DOI: 10.11116/9789461667410

ISBN: 9789461667403

Publication date: February 25, 2026

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Number of pages: 272

Number of illustrations: 50

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Funded by: KU Leuven Fund for Fair Open Access

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The importance of plant-based food fermentation and the need for analytical methods.

Food fermentation is a complex yet highly relevant topic in food science today and is very likely to remain so for years to come. It is a technology that can enhance the sensory attributes, health-related properties, and shelf life of a wide variety of foods. Typical of food fermentation processes is that they induce numerous changes in the raw material or food matrix, including various (bio)chemical conversions of food constituents. To fully unlock the potential of grain-based food fermentation processes, a robust analytical toolbox, specifically developed for fermented raw materials and foods, is essential. This edited volume, written by leading experts in the field of food science and fermentation technology and one of the outcomes of the Horizon Europe project HealthFerm, offers a comprehensive collection of chemical analytical methods, each in its distinct way suited for the characterisation of fermented grain-based raw materials and food products.

Preface

SECTION 1 INTRODUCTION

Chapter 1 HealthFerm raw materials
Wouters, A. G. B., De Bondt, Y., Polo, A., Tuccillo, F., Katina, K., Courtin, C. M.

Chapter 2 Plant-based food fermentations
De Bondt, Y., Wouters, A. G. B., Courtin, C. M.

SECTION 2 PROTEINS

Chapter 3 Determination of free amino acids and total amino acid composition in plant-based raw materials
Nygren, H., Maiorova, N., Juvonen, R., Sözer, N.

Chapter 4 Determination of the extractable protein content of (fermented) plant-based raw materials and foods
Lille, M., Janssen, F., Juvonen, R., Wouters, A. G. B.

Chapter 5 Analysis of protein degree of hydrolysis and apparent molecular weight distribution
Blontrock, E., Janssen, F., Courtin, C. M., Wouters, A. G. B.

SECTION 3 CARBOHYDRATES

Chapter 6 Analysis of total, resistant and damaged starch content and its fine structure
Herneke, A., Nilsson, K., Lambrechts, E., De Schepper, C. F., Courtin, C. M., Zamaratskaia, G., Langton, M.

Chapter 7 Analysis of mono- and disaccharides, fructans, and raffinose-family oligosaccharides
Arora, K., Tlais, A. Z. A., Viretto, C., Polo, A., Gobbetti, M., Di Cagno, R.

Chapter 8 Analysis of the content, extractability and fine structure of arabinoxylan
Lambrechts, E., De Bondt, Y., Janssen, F., Wouters, A. G. B., Courtin, C. M.

Chapter 9 Cereal β-D-Glucan : quantification, extractability, fine structure and molecular weight distribution
Boulos, S., Pandeirada, C. O., Zehnder-Wyss, O., Janssen, F., Mäkelä-Salmi, N., Nyström, L.

Chapter 10 Analysis of exopolysaccharides in fermented cereal- and pulse-based materials
Tlais, A. Z. A., Cera, S., Arora, K., Koirala, P. , Viretto, C., Polo, A., Katina, K., Maina, H. N., Gobbetti, M., Di Cagno, R.

SECTION 4 MINOR GRAIN CONSTITUENTS

Chapter 11 Analysis of the content of phytic acid, inositol phosphates and minerals
Verdonck, C., Lemmens, E., Huyskens, M., Wouters, A. G. B., Courtin, C. M., De Bondt, Y.

Chapter 12 Analysis of saponins and tannins in fermented faba bean–based raw materials
Tuccillo, F., Edelmann, M., Pöysä, M., Cera, S., Maina, N. H., Lampi, A.-M., Katina, K.

Chapter 13 Quantitative analysis of vicine and convicine in faba bean–based materials
Pöysä, M., Lampi, A.-M., Cera, S., Tuccillo, F., Katina, K.

Chapter 14 Vitamin B12 analysis in fermented cereal- and pulse-based matrices
Chamlagain, B., Edelmann, M., Cera, S., Tuccillo, F., Varmanen, P., Katina, K.

Chapter 15 Free fatty acid analysis in fermented cereal- and pulse-based matrices
Tuccillo, F., Cera, S., Maina, N.H., Lampi, A.-M., Katina, K.

SECTION 5 FERMENTATION METABOLITES AND INTERMEDIATES

Chapter 16 Quantitative analysis of organic acids in fermented food matrices
Gettemans, T., Weckx, S., De Vuyst, L.

Chapter 17 Quantitative analysis of biogenic amines in fermented food matrices
Gettemans, T., Weckx, S., De Vuyst, L.

Chapter 18 Quantitative analysis of ethanol in fermented food matrices
Gettemans, T., Weckx, S., De Vuyst, L.

Chapter 19 Quantitative analysis of sugar alcohols in fermented food matrices
Gettemans, T., Weckx, S., De Vuyst, L.

Chapter 20 Analysis of volatile compounds in (fermented) cereal- and pulse-based materials
Tuccillo, F., Cera, S., Maina, N. H., Lampi, A.-M., Katina, K.

Arno G. B. WoutersORCID icon

Arno G.B. Wouters is associate professor in Food Chemistry at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and deputy work package leader in the Horizon Europe project HealthFerm.

Yamina De Bondt

Yamina De Bondt is postdoctoral researcher at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and part of the management team for the Horizon Europe project HealthFerm.

Kati Katina

Kati Katina is full professor at the Department of Food and Nutrition at the University of Helsinki and is work package leader in the Horizon Europe project HealthFerm.

Christophe M. Courtin

Christophe M. Courtin is full professor in Food Biochemistry at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and coordinator of the Horizon Europe project HealthFerm.

This work is a comprehensive compendium of state of the art methods for analysis of cereals and legumes and the fermented products that can be made from them. It will be a great resource for many scholars in the field. - Andrew Ross, Oregon State University

Frequently Asked Questions

What is "Characterisation of Fermented Grain-based Raw Materials and Foods" about?

The importance of plant-based food fermentation and the need for analytical methods.

Food fermentation is a complex yet highly relevant topic in food science today and is very likely to remain so for years to come. It is a technology that can enhance the sensory attributes, health-related properties, and shelf life of a wide variety of foods. Typical of food fermentation processes is that they induce numerous changes in the raw material or food matrix, including various (bio)chemical conversions of food constituents. To fully unlock the potential of grain-based food fermentation processes, a robust analytical toolbox, specifically developed for fermented raw materials and foods, is essential. This edited volume, written by leading experts in the field of food science and fermentation technology and one of the outcomes of the Horizon Europe project HealthFerm, offers a comprehensive collection of chemical analytical methods, each in its distinct way suited for the characterisation of fermented grain-based raw materials and food products.

Who edited "Characterisation of Fermented Grain-based Raw Materials and Foods"?

"Characterisation of Fermented Grain-based Raw Materials and Foods" was edited by Arno G. B. Wouters, Yamina De Bondt, Kati Katina and Christophe M. Courtin.

Arno G.B. Wouters is associate professor in Food Chemistry at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and deputy work package leader in the Horizon Europe project HealthFerm.

Yamina De Bondt is postdoctoral researcher at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and part of the management team for the Horizon Europe project HealthFerm.

Kati Katina is full professor at the Department of Food and Nutrition at the University of Helsinki and is work package leader in the Horizon Europe project HealthFerm.

Christophe M. Courtin is full professor in Food Biochemistry at the Laboratory of Food Chemistry and Biochemistry of KU Leuven and coordinator of the Horizon Europe project HealthFerm.

Is "Characterisation of Fermented Grain-based Raw Materials and Foods" available Open Access?

Yes, "Characterisation of Fermented Grain-based Raw Materials and Foods" is published Open Access. It is available under a BY-NC-ND license.

Who funded the publication of "Characterisation of Fermented Grain-based Raw Materials and Foods"?

Publication of "Characterisation of Fermented Grain-based Raw Materials and Foods" was funded by KU Leuven Fund for Fair Open Access
.

What is the Table of Contents for "Characterisation of Fermented Grain-based Raw Materials and Foods"?
Preface SECTION 1 INTRODUCTION Chapter 1 HealthFerm raw materials Wouters, A. G. B., De Bondt, Y., Polo, A., Tuccillo, F., Katina, K., Courtin, C. M. Chapter 2 Plant-based food fermentations De Bondt, Y., Wouters, A. G. B., Courtin, C. M. SECTION 2 PROTEINS Chapter 3 Determination of free amino acids and total amino acid composition in plant-based raw materials Nygren, H., Maiorova, N., Juvonen, R., Sözer, N. Chapter 4 Determination of the extractable protein content of (fermented) plant-based raw materials and foods Lille, M., Janssen, F., Juvonen, R., Wouters, A. G. B. Chapter 5 Analysis of protein degree of hydrolysis and apparent molecular weight distribution Blontrock, E., Janssen, F., Courtin, C. M., Wouters, A. G. B. SECTION 3 CARBOHYDRATES Chapter 6 Analysis of total, resistant and damaged starch content and its fine structure Herneke, A., Nilsson, K., Lambrechts, E., De Schepper, C. F., Courtin, C. M., Zamaratskaia, G., Langton, M. Chapter 7 Analysis of mono- and disaccharides, fructans, and raffinose-family oligosaccharides Arora, K., Tlais, A. Z. A., Viretto, C., Polo, A., Gobbetti, M., Di Cagno, R. Chapter 8 Analysis of the content, extractability and fine structure of arabinoxylan Lambrechts, E., De Bondt, Y., Janssen, F., Wouters, A. G. B., Courtin, C. M. Chapter 9 Cereal β-D-Glucan : quantification, extractability, fine structure and molecular weight distribution Boulos, S., Pandeirada, C. O., Zehnder-Wyss, O., Janssen, F., Mäkelä-Salmi, N., Nyström, L. Chapter 10 Analysis of exopolysaccharides in fermented cereal- and pulse-based materials Tlais, A. Z. A., Cera, S., Arora, K., Koirala, P. , Viretto, C., Polo, A., Katina, K., Maina, H. N., Gobbetti, M., Di Cagno, R. SECTION 4 MINOR GRAIN CONSTITUENTS Chapter 11 Analysis of the content of phytic acid, inositol phosphates and minerals Verdonck, C., Lemmens, E., Huyskens, M., Wouters, A. G. B., Courtin, C. M., De Bondt, Y. Chapter 12 Analysis of saponins and tannins in fermented faba bean–based raw materials Tuccillo, F., Edelmann, M., Pöysä, M., Cera, S., Maina, N. H., Lampi, A.-M., Katina, K. Chapter 13 Quantitative analysis of vicine and convicine in faba bean–based materials Pöysä, M., Lampi, A.-M., Cera, S., Tuccillo, F., Katina, K. Chapter 14 Vitamin B12 analysis in fermented cereal- and pulse-based matrices Chamlagain, B., Edelmann, M., Cera, S., Tuccillo, F., Varmanen, P., Katina, K. Chapter 15 Free fatty acid analysis in fermented cereal- and pulse-based matrices Tuccillo, F., Cera, S., Maina, N.H., Lampi, A.-M., Katina, K. SECTION 5 FERMENTATION METABOLITES AND INTERMEDIATES Chapter 16 Quantitative analysis of organic acids in fermented food matrices Gettemans, T., Weckx, S., De Vuyst, L. Chapter 17 Quantitative analysis of biogenic amines in fermented food matrices Gettemans, T., Weckx, S., De Vuyst, L. Chapter 18 Quantitative analysis of ethanol in fermented food matrices Gettemans, T., Weckx, S., De Vuyst, L. Chapter 19 Quantitative analysis of sugar alcohols in fermented food matrices Gettemans, T., Weckx, S., De Vuyst, L. Chapter 20 Analysis of volatile compounds in (fermented) cereal- and pulse-based materials Tuccillo, F., Cera, S., Maina, N. H., Lampi, A.-M., Katina, K.
What is the DOI for "Characterisation of Fermented Grain-based Raw Materials and Foods"?

The Digital Object Identifier (DOI) for "Characterisation of Fermented Grain-based Raw Materials and Foods" is 10.11116/9789461667410.

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